Westernblot试剂配制及操作步骤(最新整理)
Western blot试剂配制1.30%丙稀酰胺储存液丙稀酰胺29gN,N’-亚甲双丙稀酰胺1g双蒸水定容到100ml,经高压蒸汽灭菌的滤纸过滤避光,4℃保存于棕瓶中2.1.5 M Tris-HCl (MW=121.1)pH 8.8Tris碱90.75g港澳通行证办理流程双蒸水400ml用浓HCl调pH至8.8,加双蒸水定容至500ml。4℃冰箱保存3.1.0 M Tris pH6...
2023-09-26 24 0
Western blot试剂配制1.30%丙稀酰胺储存液丙稀酰胺29gN,N’-亚甲双丙稀酰胺1g双蒸水定容到100ml,经高压蒸汽灭菌的滤纸过滤避光,4℃保存于棕瓶中2.1.5 M Tris-HCl (MW=121.1)pH 8.8Tris碱90.75g港澳通行证办理流程双蒸水400ml用浓HCl调pH至8.8,加双蒸水定容至500ml。4℃冰箱保存3.1.0 M Tris pH6...