专利名称：SCREENING FOR BONE ANABOLIC FACTORS 发明人：Anita Vibsig Neutzskywulff,Kim
Henriksen,Morten Karsdal
sidselrasmussen申请号：US12450027
申请日：20080414
公开号：US20100093014A1
公开日：
20100415
摘要：A method for screening for modulators of the secretion by an osteoclast of a wnt or a wnt signal enhancer comprises exposing osteoclasts in culture to a compound to be screened, exposing a wnt sensitive detection system to conditioned medium from said osteoclast culture, and determining whether a wnt signal is present in said medium by assaying for wnt mediated activation of bone formation by osteoblasts or by wnt mediated activation of LRP5 and or LPR6 signalling in a cell by detection of β catenin or detection of translocation of disheveled, axin, or Frat1 to the cell membrane of said cell.
申请人：Anita Vibsig Neutzskywulff,Kim Henriksen,Morten Karsdal
地址：Frederiksvaerk DK,Hillerod DK,Copenhagen DK
国籍：DK,DK,DK
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